منابع مشابه
The biotin requirement of Micrococcus lysodeikticus.
The response of M. lyodeiktiwu to biotin or other nutrients was determined by turbidimetric measurement of growth. Five ml of test medium contained in aluminum-capped test tubes were used in each assay. Additions to 4 ml of basal medium varied the composition of the nutrient material. The basal medium (no. 1) (table 1) was stored under toluene in the refrigerator and aliquots withdrawn aseptica...
متن کاملRequirement for biotin and the function of biotin in cells in culture.
The requirement of biotin in the culture medium has been established for HeLa cells, human fibroblasts, baby hamster kidney cells (BHK) as well as polyoma-transformed BHK cells. Growth, viability, biotin content and the activities of biotin-dependent enzymes were used as criteria. Cells in culture appear to bind and internalized avidin as well as the avidin-biotin complex. Avidin seems to mimic...
متن کاملDietary Iron Requirement of Goldfish (Carassius auratus) Fry
To estimate the dietary iron requirement of common goldfish, Carassius auratus (Linnaeus, 1758), eight isoproteinous diets containing graded levels of dietary iron (FeSO4.7H2O) ranging 34.27-537.14 mg/kg were fed to triplicate groups of C. auratus fry in glass tanks. The rearing medium was groundwater passed through a reverse osmosis water treatment unit. Fish were fed 3% of their body weight p...
متن کاملBiotin as a growth requirement for Blastomyces dermatitidis.
It is only in the past few years that the vitamin nutrition of the fungi pathogenic to man has been studied intensively. Among the yeast-like forms, Candida albicana was the first shown to require biotin for growth (Burkholder, 1943; Burkholder and Moyer, 1943), and the same requirement was later reported for Hiutoplasma capsulatum (Salvin, 1949a). The first observation of absolute biotin-defic...
متن کاملPseudo-immunolabelling with the avidin-biotin-peroxidase complex (ABC) due to the presence of endogenous biotin in retinal Müller cells of goldfish and salamander.
Immunodetection techniques are dependent on enzyme-protein conjugates for the visualisation of antigen-antibody complexes. One of the most widely used is the avidin-biotin-peroxidase complex (ABC) method. The present study demonstrates that direct treatment of goldfish and salamander retinal sections with ABC, followed by an incubation with the chromogenic substrate 3,3-diaminobenzidine tetrahy...
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ژورنال
عنوان ژورنال: NIPPON SUISAN GAKKAISHI
سال: 1967
ISSN: 1349-998X,0021-5392
DOI: 10.2331/suisan.33.448